Hotspot

rpl20-rps12

LSC · 70874-71659 · 786 bp

Back to Tiarella

Pi 0.00254

MarkerSeek score 61.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 3
pi: 0.00254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCCGAAAGCTTGATGCAAAT	AAATGTCACGAAATCCCCCG	45.0	50.0	57.3	58.5	929-944 bp	1.0	71.2
rpl20-rps12_p2	CCCGAAAGCTTGATGCAAAT	CAGCGACGAGGAACATGTAC	45.0	55.0	57.3	58.7	892-907 bp	1.0	69.7
rpl20-rps12_p3	CCCGAAAGCTTGATGCAAAT	AGCGACGAGGAACATGTACT	45.0	50.0	57.3	58.8	891-906 bp	1.0	68.9
rpl20-rps12_p4	CCCGAAAGCTTGATGCAAAT	TCAGCGACGAGGAACATGTA	45.0	50.0	57.3	58.8	893-908 bp	1.0	68.9
rpl20-rps12_p5	CCCGAAAGCTTGATGCAAAT	GCGACGAGGAACATGTACTA	45.0	50.0	57.3	57.2	890-905 bp	1.0	68.8