Hotspot

psaA-ycf3

LSC · 43939-44586 · 648 bp

Back to Tiarella

Pi 0.00414

MarkerSeek score 62.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 4
pi: 0.00414
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	826-829 bp	1.0	79.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	755-758 bp	1.0	78.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	759-762 bp	1.0	78.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	822-825 bp	1.0	77.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	824-827 bp	1.0	77.8