Hotspot

psbZ-trnG-UCC

LSC · 37848-38559 · 712 bp

Back to Tiarella

Pi 0.00758

MarkerSeek score 64.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 8
pi: 0.00758
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	870-911 bp	1.0	77.0
psbZ-trnG-UCC_p2	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	871-912 bp	1.0	75.7
psbZ-trnG-UCC_p3	GTATTTGCTTCTCCTGACGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	873-914 bp	1.0	72.1
psbZ-trnG-UCC_p4	TGTATTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	874-915 bp	1.0	72.0
psbZ-trnG-UCC_p5	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	879-920 bp	1.0	70.6