Hotspot

trnS-UGA-psbZ

LSC · 37299-37658 · 360 bp

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Pi 0
MarkerSeek score 54.00
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
360
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAACCACTCAGCCATCTCTC CAACCGTCAGGAGAAGCAAA 55.0 50.0 58.0 58.4 457-479 bp 1.0 80.3
trnS-UGA-psbZ_p2 GAGCTATCAACCACTCAGCC CAACCGTCAGGAGAAGCAAA 55.0 50.0 58.1 58.4 464-486 bp 1.0 80.0
trnS-UGA-psbZ_p3 GGAGCTATCAACCACTCAGC CAACCGTCAGGAGAAGCAAA 55.0 50.0 58.1 58.4 465-487 bp 1.0 80.0
trnS-UGA-psbZ_p4 CAAGACCGGAGCTATCAACC CAACCGTCAGGAGAAGCAAA 55.0 50.0 58.1 58.4 472-494 bp 1.0 79.9
trnS-UGA-psbZ_p5 CAACCACTCAGCCATCTCTC AACCGTCAGGAGAAGCAAAT 55.0 45.0 58.0 57.4 456-478 bp 1.0 79.0