Hotspot

psbM-trnD-GUC

LSC · 30715-31885 · 1171 bp

Back to Tiarella

Pi 0.00399
MarkerSeek score 62.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1171
variable_sites
7
pi
0.00399
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1282-1314 bp 1.0 59.6
psbM-trnD-GUC_p2 AGGAACTAGAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.4 58.5 1289-1321 bp 1.0 56.5
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.8 57.0 1283-1315 bp 1.0 55.5
psbM-trnD-GUC_p4 GGAACTAGAATGAACAGTGCAG TTCAATTGGTCAGAGCACCG 45.5 50.0 57.1 58.5 1288-1320 bp 1.0 54.0
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.8 57.8 1281-1313 bp 1.0 53.6