Hotspot

petN-psbM

LSC · 30009-30609 · 601 bp

Back to Tiarella

Pi 0.0134

MarkerSeek score 55.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 12
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CCCACCGAAGACAGAACAAA	50.0	50.0	58.2	58.0	1322-1457 bp	1.0	80.5
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	GTAAAGGTCCCACCGAAGAC	50.0	55.0	58.2	57.9	1330-1465 bp	1.0	80.1
petN-psbM_p3	GGGGACACAATTCACATGGA	CCCACCGAAGACAGAACAAA	50.0	50.0	57.8	58.0	1344-1479 bp	1.0	80.0
petN-psbM_p4	AGGGGACACAATTCACATGG	CCCACCGAAGACAGAACAAA	50.0	50.0	57.8	58.0	1345-1480 bp	1.0	79.9
petN-psbM_p5	GGGGACACAATTCACATGGA	GTAAAGGTCCCACCGAAGAC	50.0	55.0	57.8	57.9	1352-1487 bp	1.0	79.6