Hotspot

trnH-GUG-psbA

LSC · 77-417 · 341 bp

Back to Tiarella

Pi 0.0277
MarkerSeek score 64.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
341
variable_sites
14
pi
0.0277
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CCACTGCCTTATCCACTTGG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.2 57.9 410-418 bp 1.0 81.6
trnH-GUG-psbA_p2 CCACTGCCTTATCCACTTGG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.2 57.9 413-421 bp 1.0 81.5
trnH-GUG-psbA_p3 CCACTGCCTTATCCACTTGG TAGACCTAGCTGCTGTCGAA 55.0 50.0 58.2 57.9 406-414 bp 1.0 81.1
trnH-GUG-psbA_p4 CCACTGCCTTATCCACTTGG ATGCATGAACGTAACGCTCA 55.0 45.0 58.2 58.3 437-445 bp 1.0 79.6
trnH-GUG-psbA_p5 CCACTGCCTTATCCACTTGG TGCATGAACGTAACGCTCAT 55.0 45.0 58.2 58.3 436-444 bp 1.0 79.6