Hotspot

petD

LSC · 75245-76475 · 1231 bp

Back to Thyrsostachys

Pi 0.00244

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1231
variable_sites: 3
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACGAAAGAAAATAAGGCGGA	CACGGTACTTCACGGTTGAA	40.0	50.0	55.4	58.1	1293 bp	1.0	58.0
petD_p2	ACGAAAGAAAATAAGGCGGA	CCTATGCACGGTACTTCACG	40.0	55.0	55.4	58.4	1299 bp	1.0	55.7
petD_p3	ACGAAAGAAAATAAGGCGGA	CTATGCACGGTACTTCACGG	40.0	55.0	55.4	58.4	1298 bp	1.0	55.7
petD_p4	ACGAAAGAAAATAAGGCGGAT	CACGGTACTTCACGGTTGAA	38.1	50.0	56.0	58.1	1293 bp	1.0	54.6
petD_p5	ACGAAAGAAAATAAGGCGGA	ACCTATGCACGGTACTTCAC	40.0	50.0	55.4	57.3	1300 bp	1.0	53.9