Hotspot

petA-psbJ

LSC · 62742-63725 · 984 bp

Back to Thyrsostachys

Pi 0.0061

MarkerSeek score 62.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 6
pi: 0.0061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCGTCCAAGGCCTTTTATTC	TCCTCTTTGGCTGATAGGTACT	50.0	45.5	57.4	58.0	1194 bp	1.0	59.9
petA-psbJ_p2	GCGTCCAAGGCCTTTTATTC	CCTCTTTGGCTGATAGGTACTG	50.0	50.0	57.4	57.9	1193 bp	1.0	59.5
petA-psbJ_p3	GCGTCCAAGGCCTTTTATTC	TTCCTCTTTGGCTGATAGGT	50.0	45.0	57.4	55.8	1195 bp	1.0	58.6
petA-psbJ_p4	GCGTCCAAGGCCTTTTATTC	ACGGTTCATATTCTGGATTGGG	50.0	45.5	57.4	58.2	1129 bp	1.0	58.6
petA-psbJ_p5	GCGTCCAAGGCCTTTTATTC	CGGTTCATATTCTGGATTGGGT	50.0	45.5	57.4	58.2	1128 bp	1.0	58.6