Hotspot

rbcL-accD

LSC · 58146-58641 · 496 bp

Back to Thyrsostachys

Pi 0.0161

MarkerSeek score 52.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 8
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GATCATATCCACGCCGGTAC	ATAAGGATATCCCCCAGCCC	55.0	55.0	58.0	58.0	1230 bp	1.0	80.5
rbcL-accD_p2	GGCTTCAGGGGGTATTCATG	ATAAGGATATCCCCCAGCCC	55.0	55.0	58.0	58.0	1069 bp	1.0	80.5
rbcL-accD_p3	ATATCCACGCCGGTACAGTA	ATAAGGATATCCCCCAGCCC	50.0	55.0	58.0	58.0	1226 bp	1.0	80.5
rbcL-accD_p4	GAGCTGTATTTGCGAGGGAA	ATAAGGATATCCCCCAGCCC	50.0	55.0	58.0	58.0	1442 bp	1.0	80.3
rbcL-accD_p5	GATCATATCCACGCCGGTAC	CAGCCCCAGTATACCGAAAC	55.0	55.0	58.0	58.1	1216 bp	1.0	80.3