Hotspot

trnF-GAA-ndhJ

LSC · 49829-50410 · 582 bp

Back to Thyrsostachys

Pi 0.00516

MarkerSeek score 61.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 3
pi: 0.00516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	691-692 bp	1.0	79.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	681-682 bp	1.0	78.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCCACGCCTTAAACGTATCC	50.0	50.0	58.1	57.7	718-719 bp	1.0	77.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGCCTTAAACGTATCCT	50.0	50.0	58.1	57.7	717-718 bp	1.0	77.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	722-723 bp	1.0	76.9