Hotspot

trnG-UCC-trnT-GGU

LSC · 13284-15926 · 2643 bp

Back to Thyrsostachys

Pi 0.00802

MarkerSeek score 61.80

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 2643
variable_sites: 21
pi: 0.00802
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	CATCGTTAGCTTGGAAGGCT	CCATGGCATTACTCTACCACC	50.0	52.4	58.0	58.1	2831-2855 bp	1.0	71.8
trnG-UCC-trnT-GGU_p2	CGTTAGCTTGGAAGGCTAGG	CCATGGCATTACTCTACCACC	55.0	52.4	58.1	58.1	2828-2852 bp	1.0	71.6
trnG-UCC-trnT-GGU_p3	CATCGTTAGCTTGGAAGGCT	GACTTACGTCTTACCATGGCA	50.0	47.6	58.0	57.8	2844-2868 bp	1.0	71.1
trnG-UCC-trnT-GGU_p4	CATCGTTAGCTTGGAAGGCT	TGACTTACGTCTTACCATGGC	50.0	47.6	58.0	57.8	2845-2869 bp	1.0	71.1
trnG-UCC-trnT-GGU_p5	CGTTAGCTTGGAAGGCTAGG	GACTTACGTCTTACCATGGCA	55.0	47.6	58.1	57.8	2841-2865 bp	1.0	70.9