primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.440798	91	1152	1159-1159	1.000000	68.6
psbA_p2	psbA	2	TTCTGGCTTTCCTTCCTTCA	AATACGTGTGCTTGGGAGTC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.440798	91	1152	1160-1160	1.000000	68.6
psbA_p3	psbA	3	TCTGGCTTTCCTTCCTTCAAA	AATACGTGTGCTTGGGAGTC	21	20	42.9	50.0	57.4	57.9	0.0	0.0	1.688204	91	1152	1159-1159	1.000000	66.6
psbA_p4	psbA	4	TTCTGGCTTTCCTTCCTTCAA	AATACGTGTGCTTGGGAGTC	21	20	42.9	50.0	57.4	57.9	0.0	0.0	1.688204	91	1152	1160-1160	1.000000	66.6
psbA_p5	psbA	5	TTTCTGGCTTTCCTTCCTTCA	AATACGTGTGCTTGGGAGTC	21	20	42.9	50.0	57.4	57.9	0.0	0.0	1.688204	91	1152	1161-1161	1.000000	66.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5616	6663	1130-1137	1.000000	78.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5616	6663	1141-1148	1.000000	78.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5616	6663	1134-1141	1.000000	77.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5616	6663	1145-1152	1.000000	76.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5616	6663	1136-1143	1.000000	75.6
trnG-UCC-trnT-GGU_p1	trnG-UCC-trnT-GGU	1	CATCGTTAGCTTGGAAGGCT	CCATGGCATTACTCTACCACC	20	21	50.0	52.4	58.0	58.1	0.0	0.0	1.178643	13284	15926	2831-2855	1.000000	71.8
trnG-UCC-trnT-GGU_p2	trnG-UCC-trnT-GGU	2	CGTTAGCTTGGAAGGCTAGG	CCATGGCATTACTCTACCACC	20	21	55.0	52.4	58.1	58.1	0.0	0.0	1.207480	13284	15926	2828-2852	1.000000	71.6
trnG-UCC-trnT-GGU_p3	trnG-UCC-trnT-GGU	3	CATCGTTAGCTTGGAAGGCT	GACTTACGTCTTACCATGGCA	20	21	50.0	47.6	58.0	57.8	0.0	13.0	1.270905	13284	15926	2844-2868	1.000000	71.1
trnG-UCC-trnT-GGU_p4	trnG-UCC-trnT-GGU	4	CATCGTTAGCTTGGAAGGCT	TGACTTACGTCTTACCATGGC	20	21	50.0	47.6	58.0	57.8	0.0	13.0	1.270905	13284	15926	2845-2869	1.000000	71.1
trnG-UCC-trnT-GGU_p5	trnG-UCC-trnT-GGU	5	CGTTAGCTTGGAAGGCTAGG	GACTTACGTCTTACCATGGCA	20	21	55.0	47.6	58.1	57.8	0.0	13.0	1.299742	13284	15926	2841-2865	1.000000	70.9
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	18358	19093	898-898	1.000000	67.7
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	21	20	42.9	60.0	57.6	58.3	0.0	0.0	1.689144	18358	19093	842-842	1.000000	66.7
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTATGGGGGAGGAGTGGA	21	20	42.9	55.0	57.6	58.4	0.0	0.0	1.779576	18358	19093	841-841	1.000000	66.0
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	56.1	58.2	0.0	0.0	2.081320	18358	19093	898-898	1.000000	63.5
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	AGTAGTATGGGGGAGGAGTG	21	20	42.9	55.0	57.6	57.2	0.0	0.0	2.184882	18358	19093	843-843	1.000000	62.7
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	19184	20083	949-954	1.000000	70.7
petN-trnC-GCA_p2	petN-trnC-GCA	2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	20	20	50.0	55.0	58.2	59.1	0.0	4.3	1.260665	19184	20083	951-956	1.000000	70.3
petN-trnC-GCA_p3	petN-trnC-GCA	3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.3	58.1	0.0	0.0	1.334774	19184	20083	1005-1010	1.000000	69.6
petN-trnC-GCA_p4	petN-trnC-GCA	4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	20	20	60.0	55.0	58.3	59.1	0.0	4.3	1.391037	19184	20083	1007-1012	1.000000	69.2
petN-trnC-GCA_p5	petN-trnC-GCA	5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.4	58.1	0.0	0.0	1.425206	19184	20083	1006-1011	1.000000	68.9
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20155	21324	1254-1267	1.000000	77.4
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	20155	21324	1253-1266	1.000000	77.4
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	20	20	50.0	45.0	58.1	57.6	14.6	0.0	0.442790	20155	21324	1318-1331	1.000000	77.0
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20155	21324	1317-1330	1.000000	76.9
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	20	20	50.0	50.0	58.1	58.4	14.6	0.0	0.457296	20155	21324	1319-1332	1.000000	76.9
rpoC2_p1	rpoC2	1	CTGGTCGAATTAGGGGAAGC	TCGATTCAACAAGGTGCCAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.070808	28718	28837	2211-2253	1.000000	79.7
rpoC2_p2	rpoC2	2	TTCGCTCCACAAGGATCAAG	GGGCTCCCATATTCGTCTTC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.270482	28718	28837	723-765	1.000000	78.4
rpoC2_p3	rpoC2	3	AATCATGTCCAACGGCCATT	GGGCTCCCATATTCGTCTTC	20	20	45.0	55.0	58.1	57.8	2.2	0.0	0.320037	28718	28837	561-603	1.000000	78.1
rpoC2_p4	rpoC2	4	TTCGCTCCACAAGGATCAAG	ACCAATCCACCTAGTCGACT	20	20	50.0	50.0	57.9	57.8	0.0	23.7	0.332446	28718	28837	1020-1062	1.000000	77.7
rpoC2_p5	rpoC2	5	TTCGCTCCACAAGGATCAAG	TTAGGGATGAGGATCTGGGC	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.364487	28718	28837	944-986	1.000000	77.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.175689	46018	46623	782-793	1.000000	79.7
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.0	57.8	0.0	5.6	0.217004	46018	46623	732-743	1.000000	79.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.571186	46018	46623	734-745	1.000000	76.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTTTCCCCTGAGGTTGTCG	CAGTTCCAATGCTACGCCTT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.571186	46018	46623	735-746	1.000000	76.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	20	20	50.0	50.0	58.0	58.6	0.0	5.6	0.575356	46018	46623	731-742	1.000000	76.6
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49829	50410	691-692	1.000000	79.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	49829	50410	681-682	1.000000	78.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TCCACGCCTTAAACGTATCC	20	20	50.0	50.0	58.1	57.7	0.0	3.2	0.350307	49829	50410	718-719	1.000000	77.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CCACGCCTTAAACGTATCCT	20	20	50.0	50.0	58.1	57.7	0.0	3.2	0.356647	49829	50410	717-718	1.000000	77.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.448485	49829	50410	722-723	1.000000	76.9
rbcL-accD_p1	rbcL-accD	1	GATCATATCCACGCCGGTAC	ATAAGGATATCCCCCAGCCC	20	20	55.0	55.0	58.0	58.0	1.9	18.6	0.020751	58146	58641	1230-1230	1.000000	80.5
rbcL-accD_p2	rbcL-accD	2	GGCTTCAGGGGGTATTCATG	ATAAGGATATCCCCCAGCCC	20	20	55.0	55.0	58.0	58.0	0.0	18.6	0.026038	58146	58641	1069-1069	1.000000	80.5
rbcL-accD_p3	rbcL-accD	3	ATATCCACGCCGGTACAGTA	ATAAGGATATCCCCCAGCCC	20	20	50.0	55.0	58.0	58.0	1.9	18.6	0.027447	58146	58641	1226-1226	1.000000	80.5
rbcL-accD_p4	rbcL-accD	4	GAGCTGTATTTGCGAGGGAA	ATAAGGATATCCCCCAGCCC	20	20	50.0	55.0	58.0	58.0	0.0	18.6	0.037096	58146	58641	1442-1442	1.000000	80.3
rbcL-accD_p5	rbcL-accD	5	GATCATATCCACGCCGGTAC	CAGCCCCAGTATACCGAAAC	20	20	55.0	55.0	58.0	58.1	1.9	0.0	0.058365	58146	58641	1216-1216	1.000000	80.3
petA-psbJ_p1	petA-psbJ	1	GCGTCCAAGGCCTTTTATTC	TCCTCTTTGGCTGATAGGTACT	20	22	50.0	45.5	57.4	58.0	17.6	0.0	2.566098	62742	63725	1194-1194	1.000000	59.9
petA-psbJ_p2	petA-psbJ	2	GCGTCCAAGGCCTTTTATTC	CCTCTTTGGCTGATAGGTACTG	20	22	50.0	50.0	57.4	57.9	17.6	0.0	2.627055	62742	63725	1193-1193	1.000000	59.5
petA-psbJ_p3	petA-psbJ	3	GCGTCCAAGGCCTTTTATTC	TTCCTCTTTGGCTGATAGGT	20	20	50.0	45.0	57.4	55.8	17.6	0.0	2.739592	62742	63725	1195-1195	1.000000	58.6
petA-psbJ_p4	petA-psbJ	4	GCGTCCAAGGCCTTTTATTC	ACGGTTCATATTCTGGATTGGG	20	22	50.0	45.5	57.4	58.2	17.6	0.0	2.741176	62742	63725	1129-1129	1.000000	58.6
petA-psbJ_p5	petA-psbJ	5	GCGTCCAAGGCCTTTTATTC	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	57.4	58.2	17.6	0.0	2.741176	62742	63725	1128-1128	1.000000	58.6
clpP-psbB_p1	clpP-psbB	1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	20	20	50.0	45.0	57.9	58.0	0.0	12.1	0.117743	70639	71150	643-644	1.000000	79.7
clpP-psbB_p2	clpP-psbB	2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	20	20	50.0	45.0	58.1	58.0	0.0	12.1	0.138185	70639	71150	646-647	1.000000	79.5
clpP-psbB_p3	clpP-psbB	3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	20	20	50.0	45.0	57.8	58.0	3.6	12.1	0.185464	70639	71150	603-604	1.000000	79.2
clpP-psbB_p4	clpP-psbB	4	ACCCAAGTCGCTTCTTCATC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.215947	70639	71150	675-676	1.000000	78.9
clpP-psbB_p5	clpP-psbB	5	TCAACCCAAGTCGCTTCTTC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.236390	70639	71150	678-679	1.000000	78.7
petD_p1	petD	1	ACGAAAGAAAATAAGGCGGA	CACGGTACTTCACGGTTGAA	20	20	40.0	50.0	55.4	58.1	0.0	0.0	2.767230	75245	76475	1293-1293	1.000000	58.0
petD_p2	petD	2	ACGAAAGAAAATAAGGCGGA	CCTATGCACGGTACTTCACG	20	20	40.0	55.0	55.4	58.4	0.0	0.0	3.066179	75245	76475	1299-1299	1.000000	55.7
petD_p3	petD	3	ACGAAAGAAAATAAGGCGGA	CTATGCACGGTACTTCACGG	20	20	40.0	55.0	55.4	58.4	0.0	0.0	3.066179	75245	76475	1298-1298	1.000000	55.7
petD_p4	petD	4	ACGAAAGAAAATAAGGCGGAT	CACGGTACTTCACGGTTGAA	21	20	38.1	50.0	56.0	58.1	0.0	0.0	3.192624	75245	76475	1293-1293	1.000000	54.6
petD_p5	petD	5	ACGAAAGAAAATAAGGCGGA	ACCTATGCACGGTACTTCAC	20	20	40.0	50.0	55.4	57.3	0.0	7.7	3.290216	75245	76475	1300-1300	1.000000	53.9
rpoA-rps11_p1	rpoA-rps11	1	AGTGTTTGAGTGGATCCTGC	GTGGAAGAGATGCCGCATTA	20	20	50.0	50.0	57.8	58.0	14.2	7.4	0.237569	77706	77769	221-221	1.000000	78.5
rpoA-rps11_p2	rpoA-rps11	2	AGTGTTTGAGTGGATCCTGC	TGGAAGAGATGCCGCATTAC	20	20	50.0	50.0	57.8	58.0	14.2	7.4	0.237569	77706	77769	220-220	1.000000	78.5
rpoA-rps11_p3	rpoA-rps11	3	GTGTTTGAGTGGATCCTGCT	GTGGAAGAGATGCCGCATTA	20	20	50.0	50.0	57.8	58.0	14.2	7.4	0.237569	77706	77769	220-220	1.000000	78.5
rpoA-rps11_p4	rpoA-rps11	4	GTGTTTGAGTGGATCCTGCT	TGGAAGAGATGCCGCATTAC	20	20	50.0	50.0	57.8	58.0	14.2	7.4	0.237569	77706	77769	219-219	1.000000	78.5
rpoA-rps11_p5	rpoA-rps11	5	CCTTTTCTAAGCGGGGACAA	TGGAAGAGATGCCGCATTAC	20	20	50.0	50.0	57.8	58.0	0.0	7.4	0.240642	77706	77769	301-301	1.000000	78.3
ndhF_p1	ndhF	1	AGGAGACAGGGGAATTCCTT	TGGTTTGGTGAATCGGAAACA	20	21	50.0	42.9	57.6	58.3	23.6	0.0	1.711944	104890	107109	2312-2312	0.500000	46.3
ndhF_p2	ndhF	2	CAGGAGACAGGGGAATTCCT	TGGTTTGGTGAATCGGAAACA	20	21	55.0	42.9	58.4	58.3	23.6	0.0	1.754583	104890	107109	2313-2313	0.500000	46.0
ndhF_p3	ndhF	3	AGGAGACAGGGGAATTCCTT	GGTTTGGTGAATCGGAAACA	20	20	50.0	45.0	57.6	56.5	23.6	0.0	1.823655	104890	107109	2311-2311	0.500000	45.4
ndhF_p4	ndhF	4	AGGAGACAGGGGAATTCCTT	TGGTTTGGTGAATCGGAAAC	20	20	50.0	45.0	57.6	56.5	23.6	0.0	1.823655	104890	107109	2312-2312	0.500000	45.4
ndhF_p5	ndhF	5	CAGGAGACAGGGGAATTCCT	GGTTTGGTGAATCGGAAACA	20	20	55.0	45.0	58.4	56.5	23.6	0.0	1.866294	104890	107109	2312-2312	0.500000	45.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108202	108919	802-802	1.000000	78.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108202	108919	801-801	1.000000	78.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108202	108919	795-795	1.000000	78.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108202	108919	794-794	1.000000	78.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	58.1	58.2	0.0	4.7	0.313714	108202	108919	815-815	1.000000	78.0