Hotspot

ycf1

SSC · 121700-127243 · 5544 bp

Back to Thymus

Pi 0.00779
MarkerSeek score 53.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5544
variable_sites
218
pi
0.00779
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCAGGCCTCAATATCGACT TGAAAATAAAACGGAATGCTT 50.0 28.6 57.9 52.6 242 bp 0.916667 42.0
ycf1_p2 TCCAGGCCTCAATATCGACT TGAAAATAAAACGGAATGCTTT 50.0 27.3 57.9 53.5 242 bp 0.916667 42.0
ycf1_p3 TCCAGGCCTCAATATCGACT TGAAAATAAAACGGAATGCTTTT 50.0 26.1 57.9 54.3 242 bp 0.916667 42.0
ycf1_p4 TCCAGGCCTCAATATCGACT ATGAAAATAAAACGGAATGCT 50.0 28.6 57.9 52.3 243 bp 0.916667 41.9
ycf1_p5 TCCAGGCCTCAATATCGACT ATGAAAATAAAACGGAATGCTT 50.0 27.3 57.9 53.3 243 bp 0.916667 41.9