Hotspot

rpoC1

LSC · 20682-23486 · 2805 bp

Pi 0.0213

MarkerSeek score 65.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2805
variable_sites: 341
pi: 0.0213
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGTAAAGAGGTCTGCATTCCT	AGGAAGGAAGCTTAATCGGA	42.9	45.0	56.3	55.9	2890-2908 bp	0.916667	40.0
rpoC1_p2	AGTTGAAGTAAAGAGGTCTGCA	AGGAAGGAAGCTTAATCGGA	40.9	45.0	57.3	55.9	2896-2914 bp	0.916667	39.7
rpoC1_p3	GAGGTCTGCATTCCTATATCGT	AGGAAGGAAGCTTAATCGGA	45.5	45.0	57.2	55.9	2884-2902 bp	0.916667	38.9
rpoC1_p4	AGTAAAGAGGTCTGCATTCCT	ACGATCTTTAGCTCTGGAACTG	42.9	45.5	56.3	57.9	2951-2963 bp	0.833333	35.7
rpoC1_p5	AGTTGAAGTAAAGAGGTCTGCA	ACGATCTTTAGCTCTGGAACTG	40.9	45.5	57.3	57.9	2957-2969 bp	0.833333	35.4