Hotspot

petA-psbJ

LSC · 62424-63417 · 994 bp

Back to Thymus

Pi 0.0038
MarkerSeek score 58.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
994
variable_sites
21
pi
0.0038
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1048-1078 bp 1.0 78.5
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1050-1080 bp 1.0 78.4
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1047-1077 bp 1.0 78.0
petA-psbJ_p4 TCGAGAAGGTTCAATTGGCC CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1049-1079 bp 1.0 78.0
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA TCCGGATTAGGTTCATCCCT 45.0 50.0 57.7 57.5 1047-1077 bp 1.0 77.6