Hotspot

ndhC-trnV-UAC

LSC · 49992-50959 · 968 bp

Back to Thymus

Pi 0.00532
MarkerSeek score 57.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
968
variable_sites
26
pi
0.00532
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1039-1120 bp 1.0 81.1
ndhC-trnV-UAC_p2 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1031-1112 bp 1.0 79.8
ndhC-trnV-UAC_p3 TTTCTCCGGCCCTTTCCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.6 57.8 1040-1121 bp 1.0 79.3
ndhC-trnV-UAC_p4 TTCTCCGGCCCTTTCCTAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.4 57.5 1038-1119 bp 1.0 78.4
ndhC-trnV-UAC_p5 TCTCCGGCCCTTTCCTAATC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.9 57.8 1038-1119 bp 1.0 77.4