Hotspot

trnK-UUU-rps16

LSC · 4227-4772 · 546 bp

Back to Thymus

Pi 0.00582
MarkerSeek score 59.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
546
variable_sites
18
pi
0.00582
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTAGCCGCACTTAAAAGCC CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.3 58.2 681-700 bp 1.0 81.8
trnK-UUU-rps16_p2 TCTAGCCGCACTTAAAAGCC CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.3 58.2 680-699 bp 1.0 81.8
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.3 58.2 676-695 bp 1.0 81.7
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.3 58.2 675-694 bp 1.0 81.7
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC TCCTTGAAAAAGGCGCTCAA 50.0 45.0 58.3 58.3 682-701 bp 1.0 81.1