primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	85	406	374-394	1.000000	88.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	85	406	388-408	1.000000	87.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	85	406	387-407	1.000000	87.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	85	406	389-409	1.000000	87.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	85	406	426-446	1.000000	86.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTAGCCGCACTTAAAAGCC	CCTTGAAAAAGGCGCTCAAC	20	20	50.0	50.0	58.3	58.2	0.0	7.6	0.494881	4227	4772	681-700	1.000000	81.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCTAGCCGCACTTAAAAGCC	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.3	58.2	0.0	7.6	0.494881	4227	4772	680-699	1.000000	81.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	CCTTGAAAAAGGCGCTCAAC	20	20	50.0	50.0	58.3	58.2	5.3	7.6	0.511849	4227	4772	676-695	1.000000	81.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.3	58.2	5.3	7.6	0.511849	4227	4772	675-694	1.000000	81.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCTAGCCGCACTTAAAAGCC	TCCTTGAAAAAGGCGCTCAA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4227	4772	682-701	1.000000	81.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5918	6832	983-1035	1.000000	83.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	20	20	45.0	55.0	58.1	57.9	8.1	0.0	0.261887	5918	6832	996-1048	1.000000	83.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAAAATCCACAACGGGTCGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.3	58.1	0.0	16.9	0.393361	5918	6832	1010-1062	1.000000	82.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCTCGCTATT	20	20	45.0	50.0	58.1	58.3	8.1	0.0	0.405161	5918	6832	1005-1057	1.000000	82.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAAAATCCACAACGGGTCGT	GTCTCGCTATTCGGAGGTTC	20	20	45.0	55.0	58.3	57.9	0.0	0.0	0.439823	5918	6832	1023-1075	1.000000	81.8
rpoC2-rpoC1_p1	rpoC2-rpoC1	1	GCCATTCCGAAGTGCTCTAT	GGCCTGTTCATATGGTATCT	20	20	50.0	45.0	57.8	54.3	0.0	2.1	3.977078	20533	20681	281-292	0.916667	47.7
rpoC2-rpoC1_p2	rpoC2-rpoC1	2	TGAAAGACCAGATTGGCTCG	GGCCTGTTCATATGGTATCT	20	20	50.0	45.0	57.9	54.3	0.0	2.1	3.829500	20533	20681	212-212	0.833333	44.6
rpoC2-rpoC1_p3	rpoC2-rpoC1	3	AAGACCAGATTGGCTCGTTC	GGCCTGTTCATATGGTATCT	20	20	50.0	45.0	57.9	54.3	0.0	2.1	3.830964	20533	20681	209-209	0.833333	44.6
rpoC2-rpoC1_p4	rpoC2-rpoC1	4	GAAAGACCAGATTGGCTCGT	GGCCTGTTCATATGGTATCT	20	20	50.0	45.0	57.9	54.3	0.0	2.1	3.830964	20533	20681	211-211	0.833333	44.6
rpoC2-rpoC1_p5	rpoC2-rpoC1	5	TATGCCATTCCGAAGTGCTC	GGCCTGTTCATATGGTATCT	20	20	50.0	45.0	58.0	54.3	6.3	2.1	3.776800	20533	20681	284-284	0.833333	44.5
rpoC1_p1	rpoC1	1	AGTAAAGAGGTCTGCATTCCT	AGGAAGGAAGCTTAATCGGA	21	20	42.9	45.0	56.3	55.9	9.2	0.0	4.720040	20682	23486	2890-2908	0.916667	40.0
rpoC1_p2	rpoC1	2	AGTTGAAGTAAAGAGGTCTGCA	AGGAAGGAAGCTTAATCGGA	22	20	40.9	45.0	57.3	55.9	0.0	0.0	4.757838	20682	23486	2896-2914	0.916667	39.7
rpoC1_p3	rpoC1	3	GAGGTCTGCATTCCTATATCGT	AGGAAGGAAGCTTAATCGGA	22	20	45.5	45.0	57.2	55.9	9.4	0.0	4.860746	20682	23486	2884-2902	0.916667	38.9
rpoC1_p4	rpoC1	4	AGTAAAGAGGTCTGCATTCCT	ACGATCTTTAGCTCTGGAACTG	21	22	42.9	45.5	56.3	57.9	9.2	0.0	4.768561	20682	23486	2951-2963	0.833333	35.7
rpoC1_p5	rpoC1	5	AGTTGAAGTAAAGAGGTCTGCA	ACGATCTTTAGCTCTGGAACTG	22	22	40.9	45.5	57.3	57.9	0.0	0.0	4.806359	20682	23486	2957-2969	0.833333	35.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AGTTCTTCTGCCAAACCCTG	TTTTGGTGATTCAGGCGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.038853	26726	27854	1304-1327	1.000000	82.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GTTCTTCTGCCAAACCCTGA	TTTTGGTGATTCAGGCGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044479	26726	27854	1303-1326	1.000000	82.9
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGTTCTTCTGCCAAACCCTG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.095246	26726	27854	1283-1306	1.000000	82.5
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GTTCTTCTGCCAAACCCTGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.100871	26726	27854	1282-1305	1.000000	82.5
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GCCAAACCCTGATCGATGAA	TTTTGGTGATTCAGGCGACA	20	20	50.0	45.0	58.2	58.0	0.5	0.0	0.275542	26726	27854	1295-1318	1.000000	81.0
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACT	20	21	50.0	42.9	58.2	56.4	0.0	0.0	2.762471	28176	29151	1105-1126	1.000000	61.6
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCT	20	20	50.0	45.0	58.2	55.4	0.0	0.0	2.781070	28176	29151	1112-1133	1.000000	61.4
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCTT	20	21	50.0	42.9	58.2	56.2	0.0	0.0	2.973013	28176	29151	1112-1133	1.000000	59.8
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TTCATTCTAGTTCCTACCGCT	20	21	50.0	42.9	58.2	56.2	0.0	0.0	2.973013	28176	29151	1113-1134	1.000000	59.8
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACTT	20	22	50.0	40.9	58.2	57.1	0.0	0.0	3.037844	28176	29151	1105-1126	1.000000	59.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30194	30757	699-718	1.000000	82.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	30194	30757	678-697	1.000000	82.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	30194	30757	675-694	1.000000	82.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30194	30757	661-680	1.000000	82.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	30194	30757	696-715	1.000000	81.6
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.244113	41106	41870	877-882	1.000000	81.6
psaA-ycf3_p2	psaA-ycf3	2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.338015	41106	41870	895-900	1.000000	80.8
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.9	57.7	4.1	19.9	0.390477	41106	41870	950-955	1.000000	80.2
psaA-ycf3_p4	psaA-ycf3	4	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	58.2	57.7	0.0	19.9	0.484379	41106	41870	968-973	1.000000	79.3
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	20	20	50.0	50.0	57.9	58.5	4.1	3.4	0.581718	41106	41870	958-963	1.000000	78.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46087	46762	710-745	1.000000	84.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46087	46762	742-777	1.000000	83.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46087	46762	704-739	1.000000	83.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46087	46762	736-771	1.000000	82.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.5	58.2	5.5	10.2	0.748957	46087	46762	702-737	1.000000	81.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.605515	49992	50959	1039-1120	1.000000	81.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.6	57.8	0.0	0.0	0.766291	49992	50959	1031-1112	1.000000	79.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTTCTCCGGCCCTTTCCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.6	57.8	0.0	0.0	0.833014	49992	50959	1040-1121	1.000000	79.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTCTCCGGCCCTTTCCTAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.4	57.5	0.0	0.0	0.949287	49992	50959	1038-1119	1.000000	78.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCTCCGGCCCTTTCCTAATC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.9	57.8	0.0	0.0	1.068225	49992	50959	1038-1119	1.000000	77.4
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62424	63417	1048-1078	1.000000	78.5
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62424	63417	1050-1080	1.000000	78.4
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62424	63417	1047-1077	1.000000	78.0
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62424	63417	1049-1079	1.000000	78.0
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	62424	63417	1047-1077	1.000000	77.6
infA-rps8_p1	infA-rps8	1	CGTTGGGAAGCGATTCAGTA	GAAGGAATCGGCGGAGAAAT	20	20	50.0	50.0	58.0	58.0	16.2	7.2	0.049656	78863	78989	213-222	1.000000	86.9
infA-rps8_p2	infA-rps8	2	CGTTGGGAAGCGATTCAGTA	ATCGGGAAGCTCGACTAGAA	20	20	50.0	50.0	58.0	57.9	16.2	0.0	0.058815	78863	78989	230-239	1.000000	86.3
infA-rps8_p3	infA-rps8	3	CGTTGGGAAGCGATTCAGTA	TAGAAGGAATCGGCGGAGAA	20	20	50.0	50.0	58.0	58.2	16.2	7.2	0.233018	78863	78989	215-224	1.000000	85.4
infA-rps8_p4	infA-rps8	4	CGTTGGGAAGCGATTCAGTA	GGGAAGCTCGACTAGAAGGA	20	20	50.0	55.0	58.0	58.2	16.2	0.0	0.239353	78863	78989	227-236	1.000000	85.0
infA-rps8_p5	infA-rps8	5	CGTTGGGAAGCGATTCAGTA	AGATCGGGAAGCTCGACTAG	20	20	50.0	55.0	58.0	58.4	16.2	0.0	0.404559	78863	78989	232-241	1.000000	83.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGAAAGGTTATTGGGCGG	GATGATATGCCATGCCGCTA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.181092	111347	112072	914-945	1.000000	84.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGGAAAGGTTATTGGGCGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.276949	111347	112072	861-892	1.000000	83.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGAAAGGTTATTGGGCGG	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.337676	111347	112072	854-885	1.000000	83.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGAAAGGTTATTGGGCGG	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.348967	111347	112072	860-891	1.000000	82.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGAAAGGTTATTGGGCGG	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.348967	111347	112072	859-890	1.000000	82.9
ycf1_p1	ycf1	1	TCCAGGCCTCAATATCGACT	TGAAAATAAAACGGAATGCTT	20	21	50.0	28.6	57.9	52.6	0.0	0.0	6.503638	123021	123140	242-242	0.916667	42.0
ycf1_p2	ycf1	2	TCCAGGCCTCAATATCGACT	TGAAAATAAAACGGAATGCTTT	20	22	50.0	27.3	57.9	53.5	0.0	0.0	6.615075	123021	123140	242-242	0.916667	42.0
ycf1_p3	ycf1	3	TCCAGGCCTCAATATCGACT	TGAAAATAAAACGGAATGCTTTT	20	23	50.0	26.1	57.9	54.3	0.0	0.0	6.802981	123021	123140	242-242	0.916667	42.0
ycf1_p4	ycf1	4	TCCAGGCCTCAATATCGACT	ATGAAAATAAAACGGAATGCT	20	21	50.0	28.6	57.9	52.3	0.0	0.0	6.738572	123021	123140	243-243	0.916667	41.9
ycf1_p5	ycf1	5	TCCAGGCCTCAATATCGACT	ATGAAAATAAAACGGAATGCTT	20	22	50.0	27.3	57.9	53.3	0.0	0.0	6.836252	123021	123140	243-243	0.916667	41.9