Hotspot

rpl16

LSC · 82713-84195 · 1483 bp

Back to Thymelaea

Pi 0.0333

MarkerSeek score 63.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1483
variable_sites: 49
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCGGATCTTTCCAGAGGTGT	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.1	58.0	2102-2107 bp	1.0	82.7
rpl16_p2	CAATATGAGCATACCGGCGA	CAAGTACAAATTGCAGGGCG	50.0	50.0	57.9	58.0	2041-2046 bp	1.0	82.6
rpl16_p3	ATGAGCATACCGGCGATTAC	CAAGTACAAATTGCAGGGCG	50.0	50.0	57.9	58.0	2037-2042 bp	1.0	82.6
rpl16_p4	ATTTTTGGGCTCCGCTGTTA	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.1	58.0	1990-1995 bp	1.0	82.6
rpl16_p5	TTTTTGGGCTCCGCTGTTAT	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.1	58.0	1989-1994 bp	1.0	82.6