Hotspot

ndhF

SSC · 127789-130041 · 2253 bp

Back to Thymelaea

Pi 0.0408

MarkerSeek score 66.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 92
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TAGGAATAGGCGATGGGGAA	AAAAAGCTTTCAACGCTGCC	50.0	45.0	57.6	58.4	2804-2847 bp	1.0	78.2
ndhF_p2	AGGAATAGGCGATGGGGAAA	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.5	58.4	2803-2846 bp	1.0	77.7
ndhF_p3	TAGGAATAGGCGATGGGGAA	CTGCCCGATACCCTTTCTTT	50.0	50.0	57.6	57.6	2789 bp	0.5	53.4
ndhF_p4	AGGAATAGGCGATGGGGAAA	CTGCCCGATACCCTTTCTTT	50.0	50.0	58.5	57.6	2788 bp	0.5	52.8
ndhF_p5	GGAATAGGCGATGGGGAAAT	CTGCCCGATACCCTTTCTTT	50.0	50.0	57.4	57.6	2787 bp	0.5	51.9