Hotspot

accD

LSC · 59723-61339 · 1617 bp

Back to Thymelaea

Pi 0.0222

MarkerSeek score 60.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1617
variable_sites: 35
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATGTAAATATAGGCAAGCTCT	ACTCCAACTACCTTTTCCATACA	33.3	39.1	52.1	57.2	1644-1683 bp	1.0	43.7
accD_p2	ATGTAAATATAGGCAAGCTCT	ACTAACTCCAACTACCTTTTCCA	33.3	39.1	52.1	57.1	1648-1687 bp	1.0	43.7
accD_p3	ATGTAAATATAGGCAAGCTCT	AACTCCAACTACCTTTTCCA	33.3	40.0	52.1	54.1	1645-1684 bp	1.0	43.7
accD_p4	ATGTAAATATAGGCAAGCTCT	ACTCCAACTACCTTTTCCAT	33.3	40.0	52.1	53.8	1644-1683 bp	1.0	43.7
accD_p5	ATGTAAATATAGGCAAGCTCT	ACTCCAACTACCTTTTCCATACAA	33.3	37.5	52.1	57.8	1644-1683 bp	1.0	43.7