Hotspot

ndhF-rpl32

SSC · 130042-130436 · 395 bp

Back to Thymelaea

Pi 0.0896

MarkerSeek score 78.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 31
pi: 0.0896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGGGGAAATTGTGCGGATT	TAGGAATAGGCGATGGGGAA	45.0	50.0	58.1	57.6	1447-1457 bp	1.0	83.4
ndhF-rpl32_p2	AGGGGAAATTGTGCGGATTT	TAGGAATAGGCGATGGGGAA	45.0	50.0	58.1	57.6	1446-1456 bp	1.0	83.4
ndhF-rpl32_p3	AGGGGAAATTGTGCGGATTT	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.1	58.4	1235-1278 bp	1.0	82.7
ndhF-rpl32_p4	AAGGGGAAATTGTGCGGATT	CTGCCCGATACCCTTTCTTT	45.0	50.0	58.1	57.6	1221 bp	0.5	56.1
ndhF-rpl32_p5	AGGGGAAATTGTGCGGATTT	CTGCCCGATACCCTTTCTTT	45.0	50.0	58.1	57.6	1220 bp	0.5	56.1