Hotspot

rpl16-rps3

LSC · 84196-84346 · 151 bp

Back to Thymelaea

Pi 0.078

MarkerSeek score 76.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 11
pi: 0.078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	AGTTCGGACTATCTATGGGGT	45.0	47.6	57.6	57.4	229-238 bp	1.0	71.1
rpl16-rps3_p2	AAACCAACGAGTCACACACT	CAGTTCGGACTATCTATGGGG	45.0	52.4	57.6	57.1	230-239 bp	1.0	68.4
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	AGTTCGGACTATCTATGGGGT	45.0	47.6	57.1	57.4	224-233 bp	1.0	67.4
rpl16-rps3_p4	AAACCAACGAGTCACACACT	GTTCGGACTATCTATGGGGT	45.0	50.0	57.6	55.8	228-237 bp	1.0	66.6
rpl16-rps3_p5	ACCAACGAGTCACACACTAAG	AGTTCGGACTATCTATGGGGT	47.6	47.6	57.9	57.4	227-236 bp	1.0	65.6