Hotspot

trnW-CCA-trnP-UGG

LSC · 68998-69182 · 185 bp

Back to Thymelaea

Pi 0.0486
MarkerSeek score 73.50
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
185
variable_sites
9
pi
0.0486
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 253-260 bp 1.0 81.7
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 251-258 bp 1.0 81.7
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 264-271 bp 1.0 81.6
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 265-272 bp 1.0 81.6
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 254-261 bp 1.0 81.6