Hotspot

ycf3-trnS-GGA

LSC · 46879-47748 · 870 bp

Back to Thymelaea

Pi 0.0328

MarkerSeek score 73.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 28
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	AGTTGAAGGTGCGGAAAGAG	47.6	50.0	58.0	58.1	990-1014 bp	1.0	74.3
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	GTTGAAGGTGCGGAAAGAGA	47.6	50.0	58.0	58.1	989-1013 bp	1.0	74.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	930-954 bp	1.0	74.0
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	TTGAAGGTGCGGAAAGAGAG	47.6	50.0	58.0	57.8	988-1012 bp	1.0	73.9
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GTGCGGAAAGAGAGGGATTC	47.6	55.0	58.0	58.3	982-1006 bp	1.0	72.6