Hotspot

trnT-GGU-psbD

LSC · 33478-34720 · 1243 bp

Back to Thymelaea

Pi 0.0282

MarkerSeek score 68.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1243
variable_sites: 34
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1384-1390 bp	1.0	83.7
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1387-1393 bp	1.0	83.5
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1361-1367 bp	1.0	82.0
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	1399-1405 bp	1.0	81.8
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1392-1398 bp	1.0	81.7