Hotspot

rpoB-trnC-GCA

LSC · 28248-29411 · 1164 bp

Back to Thymelaea

Pi 0.0309

MarkerSeek score 73.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 35
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCATTTTCATCCCCGAGCAT	CTGGGGTAAAGGGATTTGCA	50.0	50.0	58.3	57.8	1215-1235 bp	1.0	80.1
rpoB-trnC-GCA_p2	CCATTTTCATCCCCGAGCAT	TGGGGTAAAGGGATTTGCAG	50.0	50.0	58.3	57.8	1214-1234 bp	1.0	80.1
rpoB-trnC-GCA_p3	CCATTTTCATCCCCGAGCAT	ACTGGGGTAAAGGGATTTGC	50.0	50.0	58.3	57.8	1216-1236 bp	1.0	80.1
rpoB-trnC-GCA_p4	CCATTTTCATCCCCGAGCAT	GGGGTAAAGGGATTTGCAGT	50.0	50.0	58.3	57.8	1213-1233 bp	1.0	80.1
rpoB-trnC-GCA_p5	ATTCCCCCATTTTCATCCCC	TGGGGTAAAGGGATTTGCAG	50.0	50.0	57.5	57.8	1220-1240 bp	1.0	78.6