Hotspot

trnS-GCU-trnG-UCC

LSC · 8879-9611 · 733 bp

Back to Thymelaea

Pi 0.0326

MarkerSeek score 72.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 23
pi: 0.0326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	845-853 bp	1.0	77.0
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	844-852 bp	1.0	77.0
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	50.0	45.0	58.0	57.0	844-852 bp	1.0	77.0
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.0	57.0	843-851 bp	1.0	77.0
trnS-GCU-trnG-UCC_p5	CGCACAACGGATTAGCAATC	ATCAAACCGAAAGACCCCTT	50.0	45.0	57.9	57.0	859-867 bp	1.0	76.2