Hotspot

rps16-trnQ-UUG

LSC · 6237-7548 · 1312 bp

Back to Thymelaea

Pi 0.042

MarkerSeek score 75.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1312
variable_sites: 54
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.1	58.0	1402-1423 bp	1.0	83.3
rps16-trnQ-UUG_p2	CCACATCGCTTCAAACGAAG	TTTGGTCCCGCTATTCGAAG	50.0	50.0	57.8	58.0	1382-1403 bp	1.0	82.6
rps16-trnQ-UUG_p3	CAGCAAATCGACCATGTCCT	TTTGGTCCCGCTATTCGAAG	50.0	50.0	58.3	58.0	1424-1445 bp	1.0	82.3
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GTTTTGGTCCCGCTATTCGA	45.0	50.0	58.1	58.3	1404-1425 bp	1.0	81.2
rps16-trnQ-UUG_p5	ACCACATCGCTTCAAACGAA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.4	58.0	1383-1404 bp	1.0	81.2