Hotspot

rpl16

LSC · 84608-86302 · 1695 bp

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Pi 0
MarkerSeek score 58.40
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1695
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGGGCACTGCTTCTTTGATT GCAGGGTTCCCTTACAAACA 45.0 50.0 58.0 58.0 2257 bp 1.0 80.0
rpl16_p2 TTGGGCACTGCTTCTTTGAT GCAGGGTTCCCTTACAAACA 45.0 50.0 58.0 58.0 2258 bp 1.0 80.0
rpl16_p3 CGAGCAATCGCACCAAAAAT GCAGGGTTCCCTTACAAACA 45.0 50.0 58.0 58.0 2423 bp 1.0 79.8
rpl16_p4 TCTCAATTCCCGAGCAATCG GCAGGGTTCCCTTACAAACA 50.0 50.0 58.1 58.0 2433 bp 1.0 79.5
rpl16_p5 TGGGCACTGCTTCTTTGATT CGCCATTCTGCTCATCGATA 45.0 50.0 58.0 57.9 2113 bp 1.0 79.5