Hotspot

ndhK

LSC · 50905-51699 · 795 bp

Pi 0.00126

MarkerSeek score 44.80

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 795
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GCACACTGGGAGCGTAAATA	TGGGCAATGAGCTTTGATGT	50.0	45.0	58.0	58.1	1132 bp	1.0	79.4
ndhK_p2	CTTATACGACGCGACTCCAG	TGGGCAATGAGCTTTGATGT	55.0	45.0	58.0	58.1	1480 bp	1.0	79.4
ndhK_p3	GGAGAATGCCAAGATGCTCA	TGGGCAATGAGCTTTGATGT	50.0	45.0	58.0	58.1	1526 bp	1.0	79.1
ndhK_p4	GCCCCTCCACAATACGAATT	TGGGCAATGAGCTTTGATGT	50.0	45.0	57.9	58.1	1575 bp	1.0	79.1
ndhK_p5	GACGCGACTCCAGATTTCAT	TGGGCAATGAGCTTTGATGT	50.0	45.0	58.1	58.1	1473 bp	1.0	79.0