Hotspot

atpI

LSC · 15202-15945 · 744 bp

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Pi 0
MarkerSeek score 31.80
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 TCTTCTCCAACCCAGTGGAT AGTTCGATTTCTGTTAGAGGACA 50.0 39.1 58.0 57.3 854 bp 1.0 49.9
atpI_p2 TTCTTCTCCAACCCAGTGGA AGTTCGATTTCTGTTAGAGGACA 50.0 39.1 58.2 57.3 855 bp 1.0 48.7
atpI_p3 TGAAACCCCTGCATTGCTTA AGTTCGATTTCTGTTAGAGGACA 45.0 39.1 57.7 57.3 829 bp 1.0 47.8
atpI_p4 ATTGAAACCCCTGCATTGCT AGTTCGATTTCTGTTAGAGGACA 45.0 39.1 58.4 57.3 831 bp 1.0 47.4
atpI_p5 TCTTCTCCAACCCAGTGGAT AGTTCGATTTCTGTTAGAGGACAA 50.0 37.5 58.0 57.9 854 bp 1.0 46.9