Hotspot

atpF

LSC · 12556-14071 · 1516 bp

Pi 0

MarkerSeek score 58.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1516
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCTCGAAGGGTTCCCATTAG	CGGAGCGATACAAAAAGAACTC	55.0	45.5	58.0	57.8	1680-1687 bp	1.0	62.0
atpF_p2	GCTCGAAGGGTTCCCATTAG	CGGAGCGATACAAAAAGAACT	55.0	42.9	58.0	56.6	1680-1687 bp	1.0	61.2
atpF_p3	AGCTCGAAGGGTTCCCATTA	CGGAGCGATACAAAAAGAACTC	50.0	45.5	58.4	57.8	1681-1688 bp	1.0	59.0
atpF_p4	AGCTCGAAGGGTTCCCATTA	CGGAGCGATACAAAAAGAACT	50.0	42.9	58.4	56.6	1681-1688 bp	1.0	58.2
atpF_p5	GCTCGAAGGGTTCCCATTAG	CGGAGCGATACAAAAAGAAC	55.0	45.0	58.0	55.1	1680-1687 bp	1.0	57.1