Hotspot

ndhF-rpl32

SSC · 116515-117589 · 1075 bp

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Pi 0.00093
MarkerSeek score 59.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1075
variable_sites
1
pi
0.00093
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGCGGAAAAAGGGGTAT GTACGTCTTTTTGGAACTGCC 50.0 47.6 57.9 57.7 1148 bp 1.0 68.8
ndhF-rpl32_p2 GGAAGCGGAAAAAGGGGTAT TACGTCTTTTTGGAACTGCC 50.0 45.0 57.9 56.3 1147 bp 1.0 65.2
ndhF-rpl32_p3 AACTGGAAGCGGAAAAAGGG GTACGTCTTTTTGGAACTGCC 50.0 47.6 58.7 57.7 1152 bp 1.0 64.6
ndhF-rpl32_p4 ACTGGAAGCGGAAAAAGGG GTACGTCTTTTTGGAACTGCC 52.6 47.6 58.0 57.7 1151 bp 1.0 61.7
ndhF-rpl32_p5 GGAAGCGGAAAAAGGGGTAT AGTACGTCTTTTTGGAACTGC 50.0 42.9 57.9 56.8 1149 bp 1.0 61.1