Hotspot

trnS-GCU-trnG-GCC

LSC · 8685-9797 · 1113 bp

Back to Thunia

Pi 0.000899
MarkerSeek score 59.40
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1113
variable_sites
1
pi
0.000899
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1181-1182 bp 1.0 51.8
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1180-1181 bp 1.0 51.8
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1183-1184 bp 1.0 50.8
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1184-1185 bp 1.0 50.7
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1182-1183 bp 1.0 50.7