Hotspot

rps16-trnQ-UUG

LSC · 6128-7233 · 1106 bp

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Pi 0
MarkerSeek score 58.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1106
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GACCAAATCAAAATGGGGCG 50.0 50.0 57.9 58.0 1262-1263 bp 1.0 79.5
rps16-trnQ-UUG_p2 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1201-1202 bp 1.0 79.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GACCAAATCAAAATGGGGCG 45.0 50.0 58.1 58.0 1239-1240 bp 1.0 78.9
rps16-trnQ-UUG_p4 CCACAGCCAATCATGTCCTT GACCAAATCAAAATGGGGCG 50.0 50.0 58.2 58.0 1260-1261 bp 1.0 78.7
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GACCAAATCAAAATGGGGCG 50.0 50.0 58.2 58.0 1250-1251 bp 1.0 78.4