Hotspot

trnI-GAU-rrn16

IRa · 134639-134935 · 297 bp

Back to Thunbergia

Pi 0.0438

MarkerSeek score 77.70

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: IRa
length_bp: 297
variable_sites: 13
pi: 0.0438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	423 bp	1.0	78.8
trnI-GAU-rrn16_p2	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	391 bp	1.0	78.8
trnI-GAU-rrn16_p3	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	55.0	50.0	57.8	58.3	445 bp	1.0	78.6
trnI-GAU-rrn16_p4	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	413 bp	1.0	78.5
trnI-GAU-rrn16_p5	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	55.0	50.0	57.8	57.6	432 bp	1.0	78.2