Hotspot

rrn16-trnI-GAU

IRb · 101500-101796 · 297 bp

Back to Thunbergia

Pi 0.0438

MarkerSeek score 77.70

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 297
variable_sites: 13
pi: 0.0438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	423 bp	1.0	78.8
rrn16-trnI-GAU_p2	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	391 bp	1.0	78.8
rrn16-trnI-GAU_p3	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	445 bp	1.0	78.6
rrn16-trnI-GAU_p4	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	413 bp	1.0	78.5
rrn16-trnI-GAU_p5	AAGTCGTAACAAGGTAGCCG	CTATCCATGTGGCTGAGAGC	50.0	55.0	57.6	57.8	432 bp	1.0	78.2