Hotspot

rpoA-rps11

LSC · 78990-79054 · 65 bp

Back to Thunbergia

Pi 0.082

MarkerSeek score 78.60

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 5
pi: 0.082
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	AGCGCCCATAATAAAGACGT	GGATGTAACCCCTATGCCAC	45.0	55.0	57.4	57.7	216-217 bp	1.0	76.2
rpoA-rps11_p2	AGCGCCCATAATAAAGACGT	ATGGCTGTAGACCTCCGAAA	45.0	50.0	57.4	58.4	193-194 bp	1.0	75.4
rpoA-rps11_p3	AGCGCCCATAATAAAGACGT	AATGGCTGTAGACCTCCGAA	45.0	50.0	57.4	58.4	194-195 bp	1.0	75.3
rpoA-rps11_p4	AGCGCCCATAATAAAGACGT	TAATGGCTGTAGACCTCCGA	45.0	50.0	57.4	57.6	195-196 bp	1.0	75.2
rpoA-rps11_p5	AGCGCCCATAATAAAGACGT	GGCTGTAGACCTCCGAAAAA	45.0	50.0	57.4	57.5	191-192 bp	1.0	75.1