Hotspot

petD-rpoA

LSC · 77818-77975 · 158 bp

Back to Thunbergia

Pi 0.0949

MarkerSeek score 74.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 15
pi: 0.0949
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGATAAATCTCTAACTTTAGGTCT	GTGCATTCTAGAAAAACATTTCGC	29.2	37.5	52.7	57.9	241-242 bp	1.0	45.0
petD-rpoA_p2	TGATAAATCTCTAACTTTAGGTCT	TGCATTCTAGAAAAACATTTCGCA	29.2	33.3	52.7	58.2	240-241 bp	1.0	45.0
petD-rpoA_p3	TGATAAATCTCTAACTTTAGGTCTT	GTGCATTCTAGAAAAACATTTCGC	28.0	37.5	53.5	57.9	241-242 bp	1.0	45.0
petD-rpoA_p4	TGATAAATCTCTAACTTTAGGTCT	GCATTCTAGAAAAACATTTCGCA	29.2	34.8	52.7	56.7	239-240 bp	1.0	45.0
petD-rpoA_p5	TTGATAAATCTCTAACTTTAGGTCT	GTGCATTCTAGAAAAACATTTCGC	28.0	37.5	53.5	57.9	242-243 bp	1.0	44.9