Hotspot

psbT-psbN

LSC · 74397-74456 · 60 bp

Back to Thunbergia

Pi 0.133

MarkerSeek score 78.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 8
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	ATCCATTTGAGGAACACGGG	47.6	50.0	57.6	57.9	125 bp	1.0	72.9
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	TTTGAGGAACACGGGGACTA	47.6	50.0	57.6	58.3	120 bp	1.0	71.9
psbT-psbN_p3	TCGAGAACCACCTAAAGTTCC	ATCCATTTGAGGAACACGGG	47.6	50.0	57.3	57.9	126 bp	1.0	70.7
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	TTTGAGGAACACGGGGACTA	47.6	50.0	57.3	58.3	121 bp	1.0	69.7
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	GGTACGCCCTATATACCGCT	47.6	55.0	57.6	58.5	173 bp	1.0	68.3