Hotspot

ycf3-trnS-GGA

LSC · 44664-45373 · 710 bp

Back to Thunbergia

Pi 0.0377

MarkerSeek score 63.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 20
pi: 0.0377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	ATGCTTGTTGTCCCAACCAT	AACCATTTGACCCTTCACCC	45.0	50.0	58.0	58.0	1519-1675 bp	1.0	85.7
ycf3-trnS-GGA_p2	ACTTCACCTTCAACAAGCGT	AACCATTTGACCCTTCACCC	45.0	50.0	58.0	58.0	1257-1413 bp	1.0	85.7
ycf3-trnS-GGA_p3	AGGTGTAGTGCTTCTTCCCT	AACCATTTGACCCTTCACCC	50.0	50.0	58.0	58.0	1433-1589 bp	1.0	85.6
ycf3-trnS-GGA_p4	GGTACTAGTGGAGTGGGGTT	AACCATTTGACCCTTCACCC	55.0	50.0	58.1	58.0	1400-1556 bp	1.0	85.5
ycf3-trnS-GGA_p5	TTGGTACTAGTGGAGTGGGG	AACCATTTGACCCTTCACCC	55.0	50.0	58.1	58.0	1402-1558 bp	1.0	85.4