Hotspot

trnfM-CAU-rps14

LSC · 36893-37040 · 148 bp

Back to Thunbergia

Pi 0.0432
MarkerSeek score 73.70
Primer available yes
Label trnfM-CAU-rps14

Feature Metadata

label_name
trnfM-CAU-rps14
feature_type
igs
region
LSC
length_bp
148
variable_sites
6
pi
0.0432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rps14_p1 GCTACCAAACTGCTCTACCC CGTTGTTTTTCGACCGGAAG 55.0 50.0 58.0 58.0 283-292 bp 1.0 83.9
trnfM-CAU-rps14_p2 AAGGTTATGAGCCTTGCGAG CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 302-311 bp 1.0 83.6
trnfM-CAU-rps14_p3 TTTGAACCCGTGACCTCAAG CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 319-328 bp 1.0 83.3
trnfM-CAU-rps14_p4 GATGTTGCGGAGACAGGATT CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.0 58.0 337-346 bp 1.0 83.2
trnfM-CAU-rps14_p5 ACAGGATTTGAACCCGTGAC CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.1 58.0 325-334 bp 1.0 82.7