Hotspot

rpl22

Genome · 83653-84114 · 462 bp

Back to Thuja

Pi 0.0245
MarkerSeek score 63.90
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
Genome
length_bp
462
variable_sites
17
pi
0.0245
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 AAAAAGAGAGAGAAACGATAAAA GCAAGTAGTAGTATATTTGCGGC 26.1 43.5 52.4 57.7 621-639 bp 1.0 42.2
rpl22_p2 AAAAGAGAGAGAAACGATAAAAA GCAAGTAGTAGTATATTTGCGGC 26.1 43.5 52.4 57.7 620-638 bp 1.0 42.2
rpl22_p3 AAAAAGAGAGAGAAACGATAAAAA GCAAGTAGTAGTATATTTGCGGC 25.0 43.5 53.2 57.7 621-639 bp 1.0 42.2
rpl22_p4 AAAAAGAGAGAGAAACGATAAAA ATTTGCGGCATAAATGGATT 26.1 35.0 52.4 54.2 607-625 bp 1.0 42.2
rpl22_p5 AAAAGAGAGAGAAACGATAAAAA ATTTGCGGCATAAATGGATT 26.1 35.0 52.4 54.2 606-624 bp 1.0 42.2