Hotspot

matK

Genome · 1596-3113 · 1518 bp

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Pi 0.458
MarkerSeek score 72.40
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
Genome
length_bp
1518
variable_sites
38
pi
0.458
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.04
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 TGATCCTGGCAATTGCTCAA GAGGTTATCCGGGGGTTTTC 45.0 55.0 57.8 58.0 1591 bp 1.0 83.8
matK_p2 TTGATCCTGGCAATTGCTCA GAGGTTATCCGGGGGTTTTC 45.0 55.0 57.8 58.0 1592 bp 1.0 83.8
matK_p3 ATTTGGTTGGATTTCCGGGT GAGGTTATCCGGGGGTTTTC 45.0 55.0 57.7 58.0 1636 bp 1.0 82.9
matK_p4 TGATCCTGGCAATTGCTCAA GGTTATCCGGGGGTTTTCAA 45.0 50.0 57.8 57.8 1589 bp 1.0 82.4
matK_p5 TTGATCCTGGCAATTGCTCA GGTTATCCGGGGGTTTTCAA 45.0 50.0 57.8 57.8 1590 bp 1.0 82.4