Hotspot

clpP-chlN

Genome · 125480-127009 · 1530 bp

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Pi 0.0593
MarkerSeek score 80.10
Primer available yes
Label clpP-chlN

Feature Metadata

label_name
clpP-chlN
feature_type
igs
region
Genome
length_bp
1530
variable_sites
119
pi
0.0593
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-chlN_p1 CATTCCTCCACCTCGACAAG CAATCCGTCAAGGTCTCAGG 55.0 55.0 58.0 58.0 2301-2488 bp 1.0 87.9
clpP-chlN_p2 CCGCAATAGGCCCATAAACT CAATCCGTCAAGGTCTCAGG 50.0 55.0 58.0 58.0 2535-2722 bp 1.0 87.6
clpP-chlN_p3 GTCCGCAATAGGCCCATAAA CAATCCGTCAAGGTCTCAGG 50.0 55.0 58.0 58.0 2537-2724 bp 1.0 87.5
clpP-chlN_p4 TCCGCAATAGGCCCATAAAC CAATCCGTCAAGGTCTCAGG 50.0 55.0 58.0 58.0 2536-2723 bp 1.0 87.5
clpP-chlN_p5 ATTCCTCCACCTCGACAAGA CAATCCGTCAAGGTCTCAGG 50.0 55.0 58.1 58.0 2300-2487 bp 1.0 87.5