Hotspot

chlN-ycf1

Genome · 115358-116235 · 878 bp

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Pi 0.0285
MarkerSeek score 66.60
Primer available yes
Label chlN-ycf1

Feature Metadata

label_name
chlN-ycf1
feature_type
igs
region
Genome
length_bp
878
variable_sites
34
pi
0.0285
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlN-ycf1_p1 TTCTCGTATGTGCTTCTGGC AACCGCCAAGGATCCATTTT 50.0 45.0 58.0 58.1 1606-2047 bp 1.0 84.8
chlN-ycf1_p2 TTCTCGTATGTGCTTCTGGC ACCGCCAAGGATCCATTTTT 50.0 45.0 58.0 58.1 1605-2046 bp 1.0 84.8
chlN-ycf1_p3 AGTATGGGATCGGTAGTGGG AACCGCCAAGGATCCATTTT 55.0 45.0 58.0 58.1 1679-2120 bp 1.0 84.6
chlN-ycf1_p4 AGTATGGGATCGGTAGTGGG ACCGCCAAGGATCCATTTTT 55.0 45.0 58.0 58.1 1678-2119 bp 1.0 84.6
chlN-ycf1_p5 TTCTCGTATGTGCTTCTGGC TGGATGAGAAGACCACGAGT 50.0 50.0 58.0 58.1 1639-2080 bp 1.0 84.6