Hotspot

atpE-rbcL

Genome · 110659-111105 · 447 bp

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Pi 0.0098
MarkerSeek score 66.80
Primer available yes
Label atpE-rbcL

Feature Metadata

label_name
atpE-rbcL
feature_type
igs
region
Genome
length_bp
447
variable_sites
6
pi
0.0098
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-rbcL_p1 AAGAGACAAGCAATCGAGGC GCTTTGAATCCGACACTTGC 50.0 50.0 58.3 58.0 554-594 bp 1.0 81.4
atpE-rbcL_p2 GACAAGCAATCGAGGCTGAT GCTTTGAATCCGACACTTGC 50.0 50.0 58.3 58.0 550-590 bp 1.0 80.8
atpE-rbcL_p3 GCTAGAACACGACTAGAGGC GCTTTGAATCCGACACTTGC 55.0 50.0 57.5 58.0 515-555 bp 1.0 80.0
atpE-rbcL_p4 GGCTGATGTGGCTCTCAAAA GCTTTGAATCCGACACTTGC 50.0 50.0 58.5 58.0 537-577 bp 1.0 79.9
atpE-rbcL_p5 AAGAGACAAGCAATCGAGGC CGCTTTGAATCCGACACTTG 50.0 50.0 58.3 57.8 555-595 bp 1.0 79.8