Hotspot

psbN-psbT

Genome · 93343-93427 · 85 bp

Pi 0.0247

MarkerSeek score 72.30

Primer available yes

Label psbN-psbT

Feature Metadata

label_name: psbN-psbT
feature_type: igs
region: Genome
length_bp: 85
variable_sites: 3
pi: 0.0247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbT_p1	CCTTTTGAAGAGCACGAGGA	CCCCCAAAGTTCCCGATAAA	50.0	50.0	57.8	57.5	134-141 bp	1.0	80.2
psbN-psbT_p2	CCTTTTGAAGAGCACGAGGA	ATTTTCTTCCGAGAACCCCC	50.0	50.0	57.8	57.5	150-157 bp	1.0	79.8
psbN-psbT_p3	TCCTTTTGAAGAGCACGAGG	ATTTTCTTCCGAGAACCCCC	50.0	50.0	57.8	57.5	151-158 bp	1.0	79.8
psbN-psbT_p4	CGGTTATGCCCTATACACCG	CCCCCAAAGTTCCCGATAAA	55.0	50.0	57.9	57.5	186-193 bp	1.0	79.5
psbN-psbT_p5	CGGTTATGCCCTATACACCG	ATTTTCTTCCGAGAACCCCC	55.0	50.0	57.9	57.5	202-209 bp	1.0	79.2